The R Journal: article published in 2015, volume 7:1

R as an Environment for Reproducible Analysis of DNA Amplification Experiments PDF download
Stefan Rödiger, Michał Burdukiewicz, Konstantin Blagodatskikh, Michael Jahn and Peter Schierack , The R Journal (2015) 7:1, pages 127-150.

Abstract There is an ever-increasing number of applications, which use quantitative PCR (qPCR) or digital PCR (dPCR) to elicit fundamentals of biological processes. Moreover, quantitative isother mal amplification (qIA) methods have become more prominent in life sciences and point-of-care diagnostics. Additionally, the analysis of melting data is essential during many experiments. Several software packages have been developed for the analysis of such datasets. In most cases, the software is either distributed as closed source software or as monolithic block with little freedom to perform highly customized analysis procedures. We argue, among others, that R is an excellent foundation for reproducible and transparent data analysis in a highly customizable cross-platform environment. However, for novices it is often challenging to master R or learn capabilities of the vast number of packages available. In the paper, we describe exemplary workflows for the analysis of qPCR, qIA or dPCR experiments including the analysis of melting curve data. Our analysis relies entirely on R packages available from public repositories. Additionally, we provide information related to standardized and reproducible research.

Received: 2014-11-30; online 2015-06-25
CRAN packages: dpcR, kulife, MCMC.qpcr, qPCR.CT, DivMelt, qpcR, chipPCR, MBmca, RDML, RNetCDF, archivist, settings, shiny, rateratio.test
CRAN Task Views implied by cited CRAN packages: ReproducibleResearch, Spatial, SpatioTemporal, WebTechnologies
Bioconductor packages: nondetects, qpcrNorm, HTqPCR, SLqPCR, ddCt, EasyqpcR, unifiedWMWqPCR, ReadqPCR, NormqPCR

CC BY 4.0
This article is licensed under a Creative Commons Attribution 3.0 Unported license .

  author = {Stefan Rödiger and Michał Burdukiewicz and Konstantin
          Blagodatskikh and Michael Jahn and Peter Schierack},
  title = {{R as an Environment for Reproducible Analysis of DNA
          Amplification Experiments}},
  year = {2015},
  journal = {{The R Journal}},
  doi = {10.32614/RJ-2015-011},
  url = {},
  pages = {127--150},
  volume = {7},
  number = {1}